{"id":3142,"date":"2023-07-21T14:34:10","date_gmt":"2023-07-21T05:34:10","guid":{"rendered":"http:\/\/jmc.brc.riken.jp\/ja\/?p=3142"},"modified":"2025-12-18T17:12:24","modified_gmt":"2025-12-18T08:12:24","slug":"pipeline02","status":"publish","type":"page","link":"http:\/\/jmc.brc.riken.jp\/en\/technical_support\/mouse_clinic\/platform\/pipeline02","title":{"rendered":"Behavioral screen (Pipeline 2)"},"content":{"rendered":"
\"Crawley's<\/div>\n

Top page<\/a> > Technical support<\/a> > <\/span>Japan Mouse Clinic<\/a> ><\/span> <\/span>Phenotyping Platform<\/a> > <\/span><\/span><\/span><\/span><\/span><\/span><\/span>Behavioral screen (Pipeline 2)<\/span><\/p>\n\n\n\n

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Behavioral screen (Pipeline 2)<\/h2>\n\n\n\n
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P2-01: Light\/dark transition ( 6w )<\/strong>\u00a0\u00a0-\u00a0\u00a0<\/i>SOP<\/a><\/h3>\n\n\n\n
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Light\/dark transition test is carried out in order to assess anxiolytic behavior.
Light box (20x20cm; white wall) and dark box (20x20cm; black wall) are connected by guillotine door (see picture).
Latency for first entry into the light box and time spent in the light box are index of anxiety.<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n

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P2-02: Open-field test ( 7w )<\/strong>\u00a0\u00a0-\u00a0\u00a0<\/i>SOP<\/a><\/h3>\n\n\n\n
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Open-field test is carried out in order to asses spontaneous activity in novel environment.
The open-field apparatus is made from polyvinyl chloride board (40x40x32cm, see picture).
Distance traveled and % time spent in center area for 20 min were measured using CCD camera and video imaging system as indexes of locomotor activity and emotionality.<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n

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P2-03: Crawley's social interaction test ( 9w )\u00a0\u00a0-\u00a0\u00a0<\/i>SOP<\/a><\/h3>\n\n\n\n
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Social approaches are measured.
The apparatus is consisted of three chambers as shown in left photograph. A novel mouse or novel object are placed in a wire cage at the left or right corner.
Duration for exploration of the novel mouse or the novel object are measured.<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n

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P2-04: Home-cage activity test ( 10 - 11w )\u00a0\u00a0-\u00a0\u00a0<\/i>SOP<\/a><\/h3>\n\n\n\n
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Home cage activity test is assessing spontaneous locomotor activity and activity pattern in the home-cage.
Mouse activity measurement hardware system is assembled from magnetic field change detectors, data collection boxes, and PCs.
Activity counts under 12h light\/dark cycle (08:00-20:00 on) are measured for 5 days.<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n

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P2-05: Y maze ( 12w )\u00a0\u00a0-\u00a0\u00a0<\/i>SOP<\/a><\/h3>\n\n\n\n
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Locomotor activity, persistence (restriction of interest), spatial learning and memory.
Apparatus of Y maze is consist of arms which lengths are equal as illustrated in left photograph. A mouse is put into center of the apparatus. Spontaneous locomotor activity, number of entry into each arm, and alteration ratio are measured.<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n

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P2-06: Fear conditioning test ( 13w )\u00a0\u00a0-\u00a0\u00a0<\/i>SOP<\/a><\/h3>\n\n\n\n
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The contextual and cued fear conditioning test assesses the ability of mice to learn and remember.<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n

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P2-07: Prepulse inhibition test ( 14w )\u00a0\u00a0-\u00a0\u00a0<\/i>SOP<\/a><\/h3>\n\n\n\n
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Prepulse inhibition (PPI test) is generally used to examine dysfunction of sensory motor gating system. PPI is a neurological phenomenon such as weaker prestimulus (prepulse) inhibits the reaction to a subsequent strong startling stimulus (pulse). In this test, acoustic sound was used as prepulse and pulse stimuli.
The mouse is placed in the plexiglas cylinder fixed in the sound-attenuating chamber. The peak amplitude of startle response immediately after the sound is recorded by the startle monitor.
Prepulse inhibition (%) is calculated as following, (amplitude of startle response to pulse without prepulse - amplitude of startle response to pulse immediate after prepulse) \/ amplitude of startle response to pulse without prepulse x100.<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Top page > Technical support > Japan Mouse Clinic > Phenotyping Platform > Behavioral screen (Pipeline 2) Behavioral screen (Pipeline 2) P2-01: Light\/dark transition ( 6w )\u00a0\u00a0-\u00a0\u00a0SOP Light\/dark transition test is carried out in order to assess anxiolytic behavior.Light box (20x20cm; white wall) and dark box (20x20cm; black wall) are connected by guillotine door (see picture).Latency for first entry into the light box and time spent in the light box are index of anxiety. P2-02: Open-field test ( 7w )\u00a0\u00a0-\u00a0\u00a0SOP Open-field test is carried out in order to asses spontaneous activity in novel environment.The open-field apparatus is made from polyvinyl chloride board (40x40x32cm, see picture).Distance traveled and % time spent […]<\/p>\n","protected":false},"author":11,"featured_media":5790,"parent":1364,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","sns_share_botton_hide":"","vkExUnit_sns_title":"","_vk_print_noindex":"","sitemap_hide":"","vkExUnit_sitemap":"","vkExUnit_EyeCatch_disable":"","_veu_custom_css":"","veu_display_promotion_alert":"common","_exclude_from_list_pages":"","vkexunit_cta_each_option":"","vkExUnit_childPageIndex":"","vkExUnit_pageList_ancestor":"","vkExUnit_contact_enable":"","_lightning_design_setting":{"layout":"default","site_body_padding":"true"},"footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-3142","page","type-page","status-publish","has-post-thumbnail","hentry"],"veu_head_title_object":{"title":"","add_site_title":""},"_links":{"self":[{"href":"http:\/\/jmc.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3142","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/jmc.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/jmc.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/jmc.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"http:\/\/jmc.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=3142"}],"version-history":[{"count":7,"href":"http:\/\/jmc.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3142\/revisions"}],"predecessor-version":[{"id":5937,"href":"http:\/\/jmc.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3142\/revisions\/5937"}],"up":[{"embeddable":true,"href":"http:\/\/jmc.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/1364"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/jmc.brc.riken.jp\/en\/wp-json\/wp\/v2\/media\/5790"}],"wp:attachment":[{"href":"http:\/\/jmc.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=3142"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}